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软下疳作培养有什么要求?

来源:[标签:来源] | 发布时间:2011-07-26 18:59:42 | www.chinesejk.com
常用的培养基有淋球菌胎牛血清琼脂、巧克力马血琼脂,取标本时应将溃疡表面的痂皮和碎屑去掉,用灭菌拭子采取分泌物和溃疡的基底部物质。将标本接种于平皿上,划线分离。平皿放入5%~10%二氧化碳和饱和湿度的环境中,于33℃~35℃至少培养48小时看结果。杜克雷菌的菌落较小,如针尖大小,光滑,呈半球形。但菌落常呈不同的形状,可呈半透明状、浅灰色和肤黄色等。菌落的特点是紧密,如用接种环触之,几乎整个菌落在培养基上滑动。

(编辑:小徐 )

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